| ORIGINAL ARTICLE |
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| Year : 2009 | Volume
: 63
| Issue : 5 | Page : 187-194 |
Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and type 2 diabetes mellitus : A North Indian study
Hemant Kumar Bid1, Rituraj Konwar1, CG Aggarwal2, Sunaina Gautam3, Madhukar Saxena3, V Lakshma Nayak1, Monisha Banerjee3
1 Endocrinology Division, CDRI, Lucknow, India
2 Department of Medicine, Chhatrapati Sahuji Maharaj Medical University, Lucknow, India
3 Department of Zoology, University of Lucknow, India
Correspondence Address:
Monisha Banerjee
Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow - 226 007
India
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DOI: 10.4103/0019-5359.53164 PMID: 19584489
Background : The vitamin D receptor (VDR) gene is a candidate gene for susceptibility to several diseases. Studies on association between VDR polymorphisms and risk of type 2 diabetes (T2DM) in different ethnic populations are yet inconclusive. Aims : This study was conducted to evaluate association between VDR polymorphisms and genetic susceptibility to T2DM in the north Indian population. Settings and Design : One hundred clinically diagnosed T2DM patients and 160 healthy controls from the north Indian population were recruited for genetic association study. Materials and Methods : Genomic DNA was extracted from blood and genotyped for the single nucleotide polymorphism SNPs of FokI (T/C) [rs2228570], BsmI (A/G) [rs1544410] and TaqI (C/T) [rs731236] by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Statistical Analysis Used : Genotype distribution and allelic frequencies were compared between patients and controls. Mean values and odds ratios (ORs) with 95% confidence interval (CI) were calculated using SPSS software (version 15.0). Results : The genotype distribution, allele and haplotype frequencies of VDR polymorphism did not differ significantly between patients and controls. Mean age and waist-hip ratio of patients were found to be associated with VDR polymorphism. Combination studies showed FFBbtt increased the risk of T2DM in north Indians. Conclusions : Our data suggest that VDR gene polymorphism in combination of genotypes is associated with the risk of T2DM and thus requires further studies as a probable genetic risk marker for T2DM.
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